By David F. Keren
The second one variation of this recognized explication of the options and interpretations of electrophoresis and immunofixation has been completely up-to-date. Incorporating the adjustments in know-how because the first variation used to be released, the writer has made vast revisions, fullyyt changing the part on immunoelectrophoresis with a dialogue of the present means of immunofixation. nearly all of the unique illustrations were revised, and a brand new part at the interpretation of oligoclonal bans in cerebrospinal fluid has been incorporated
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Extra info for High-Resolution Electrophoresis and Immunofixation. Techniques and Interpretation
Whereas both Coomassie brilliant blue and Amido black give similar patterns, the Amido black has less background between major bands, which makes interpretation of serum and urine electrophoretic patterns more straightforward . Part of the difference is that Coomassie brilliant blue is more sensitive than Amido black and stains small protein molecules at these sites. For cerebrospinal fluid, where sensitivity can be a problem even after concentrating a sample 80-fold, we use Coomassie brilliant blue.
If you encounter one of these rare cases, please report it to the Analbuminemia Register (Dr. , or Dr. Roberta G. Reed, Mary Imogene Bassett Hospital, Cooperstown, NY 13326; telephone: 607-547-3670). Another inherited abnormality of albumin is bisalbuminemia, in which two types of albumin that have slightly different electrophoretic mobility are produced; this results in two distinct and equal peaks in the albumin region (Figure 3-5). Some 48 High-Resolution Electrophoresis and Immunofixation Figure 3-4.
Vol 1, Physiology of immunoglobulins: diagnostic and clinical aspects. New York: Liss, 1982:29-63. 22. Bush D, Keren DF. Over- and underestimation of monoclonal gammopathies by quantification of kappa and lambda containing immunoglobulins in serum. Clin Chem 1992;38:315-316. 23. Sinclair D, Ballantyne F, Shanley S, Caine E, O'Reilly D, Shenkin A. Estimation of paraproteins by immunoturbidimetry and electrophoresis followed by scanning densitometry. Ann Clin Biochem 1990;27:335-337. 24. Stemerman D, Papadea C, Martino-Saltzman D, O'Connell AC, Demaline B, Austin GE.
High-Resolution Electrophoresis and Immunofixation. Techniques and Interpretation by David F. Keren